The new energy regime and higher data-taking rate of the LHC will soon extend the possibilities of searches for yet undiscovered particles and phenomena. Final states produced via b-quark decays are often created by physics beyond the standard model; they are also favored in light standard model Higgs boson processes. High-pt B-tagging, therefore, has been and continues to be one of the most important tools in searches both at the Tevatron and at the LHC. In the first half of my presentation, I will introduce the CMS pixel detector, a component of the CMS tracking system that is vital for B-tagging, and talk about the on-going calibration efforts in its commissioning. In the second half of my presentation, I will demonstrate the use of B-tagging in physics analyses through an example of a low-mass standard model Higgs boson search perfomed in the CDF experiment at the Tevatron.